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KMID : 0384119930130020219
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Korean Journal of Clinical Pathology
1993 Volume.13 No. 2 p.219 ~ p.224
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Evaluation for Counting Reticulocytes by FACScan
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Abstract
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The conventional method of new methylene blue stain has been used conveniently in the test of reticulocyte count. Recently flowcytometry has been applied to reticulocyte count using thiazole orange that binds to RNA in red cells, and this method
gives
the results of mean channel fluorescence(MCF) in addition to routine reticulocyte count. The purposes of this study are to compare the values of reticulocyte count and MCF for adults, normal neonates, and premature/low birth weight neonates using
a
flow
cytometer(FACScan, Becton-Dickinson), and to investigate the clinical usefulness of automated reticulocyte test. When we compared reticulocyte count of flowcytometry(X) and new methylene blue method (Y) with 57 samples, the correlation
coefficient
was
0.78(Y=0.73x+0.56) and the differences between two methods were not significant. Reference values(mean±2SD) of reticulocyte count were 1.00-3.16% in adults(n=41), 1.8-6.6% in normal neonates(n=97), and 0.1-10.5% in premature/low birth weight
neonates(n=39), and values of neonates within 9 days after birth were significantly higher than those of adults. And reference values of MCF were 59.1-73.9 in adults, 52.9-95.3 in normal neonates, and 62.9-82.3 in premature/low birth weight
neonates,
values were significantly different among groups. We suggest that MCF may have clinical usefulness, especially as reticulocyte maturation index for neonate within first week, who have active erythropoiesis.
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KEYWORD
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